Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Article Title: Levetiracetam Protects Against Cognitive Impairment of Subthreshold Convulsant Discharge Model Rats by Activating Protein Kinase C (PKC)-Growth-Associated Protein 43 (GAP-43)-Calmodulin-Dependent Protein Kinase (CaMK) Signal Transduction Pathway
doi: 10.12659/MSM.913542
Figure Lengend Snippet: The primer sequences for the real-time PCR.
Article Snippet: The PVDF membranes were incubated with rabbit anti-rat NCAM polyclonal antibody (Catalogue No: GTX133217, 1: 2000, GeneTex, Inc., Alton Pkwy Irvine, CA, USA), mouse anti-rat GAP43 monoclonal antibody (Catalogue No. GTX34384, 1: 3000, GeneTex, Inc.), mouse anti-rat PSD-95 monoclonal antibody (Catalogue No. MABN68, 1: 3000, Millipore, Boston, MA, USA), mouse anti-rat CaMK II monoclonal antibody (Catalogue No. sc-5306, 1: 3000, Santa Cruz Biotech), and mouse anti-β actin monoclonal antibody (Catalogue No. GTX11003, 1: 3000, GeneTex Inc.) for 2 h at room temperature.
Techniques: